Environmental DNA Marine France Calanques 2022
Datasets
Description: Fish environmental DNA data set collected in 2022 in the Calanques National Park The eDNA samples were collected in 2022 in two locations (Moyades, M−FPA and “Impérial du large”, I-LPA), during the winter (January- February), the summer (June to August)and fall (mid-September to November) seasons, with the sampling dates depending on the weather conditions. For the M−FPA, samples were collected between Moyades island and Riou island, while for the I-LPA, they were collected on the south side of the “Impérial du large” island. To account for the existing bathymetry, in the M−FPA the samples were taken at two sampling sites with depths of 20 and 40 m, while in the I-LPA they were taken at two sites with depths of 20 and 80 m. At each site, in-situ filtration of seawater was performed using a double-head submersible pump (Subspace, Geneva, Switzerland; nominal flow of ca. 1 L/min) strapped to an underwater scooter with 2 VigiDNA 0.20 µm filtration capsules (SPYGEN, le Bourget du Lac, France), along with disposable sterile tubing. The samples were collected along two horizontal transects (up to 400 m in length) during each closed-circuit rebreather dive, enabling the filtr