Field-based eDNA analysis to detect the threatened and elusive African manatee
Datasets
Data associated to the publication "Rapid field-based detection of a threatened and elusive species with environmental DNA and CRISPR-Dx" -br- Authors: -br- Flurin Leugger, Martina Lüthi, Michel Schmidlin, Zacharias Kontarakis and Loïc Pellissier -br- Contact: -br- [email protected] [email protected] -br- Project description: -br- We collected eDNA samples in the Conkouati-Douli National Park in the Republic of Congo in 2023 in collaboration with Beauval Nature and HELP Congo. We analyzed the samples on site in the national park using CRISPR-Dx assays to detect the African manatee (*Trichechus senegalensis*). Additionally, we transported the remaining samples (buffer) to Switzerland and used a high-sensitivity protocol with CRISPR-Dx assays and a qPCR assay to verify the field-based detections. The qPCR of Hunter et al. (2018) detects the genus manatee (Trichechus). See publication for more details (doi will be added after publication). -br- Folders: -br- lateral_flow_tests_images\: -br- Images of the lateral flow tests used to analyze the eDNA extracts in the national park. File names correspond to the site names. At each site, we took two filter replicates.